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Alfred G. Redfield

Professor of Physics, Emeritus
Ph.D., University of Illinois, Urbana-Champaign, 1953
Fellow, American Academy of Arts and Sciences
Fellow, National Academy of Sciences

I am building a device that can be inserted into the top of a commercial NMR spectrometer (in our case, our shared 11.7 Tesla Varian system) in order to pneumatically raise and lower a sample to a lower magnetic field, and back to 11.7 T, in a round-trip time of 100 msec or less, in the middle of an NMR experiment. This will greatly extend the capabilities of these powerful instruments. For example, my first project will be to observe proton-to-phosphorous transfer-of-saturation (NOEs) in small DNA duplexes, an experiment impossible up to now in usefully high magnetic fields. Further details and reports can be found at my Web site.

Sample of Recent Publications

NMR Studies of the Conformal Change in Human N-p21^ras Produced by Replacement of Bound GDP with the GTP Analog GTP \gamma S (with A.-F. Miller and M.Z. Papastavros), Biochemistry 31, 10208 (1992).

An NMR Comparison of the Changes Produced by Different Guanosine 5'-triphosphate Analogs in Wild-type and Oncogene Mutant p21^ras (with A.F. Miller, and C.J. Halkides), Biochemistry 32, 7367 (1993).

Mapping the Nucleotide-Dependent Conformational Change of Human N-ras p21 in Solution by Heteronuclear-edited Proton-observed NMR Methods (with J.S. Hu), Biochemistry 32, 7367 (1993).

Characterization of the Active Site of p21 ras by Electron Spin-Echo Envelope Modulation Spectroscopy with Selective Labeling: Comparisons Between GDP and GTP Forms (with C.J. Halkides, C.T. Farrar, R.G. Larsen, and D.J. Singel), Biochemistry 33, 4019 (1994).

Development of a Field-Cycling NMR Method to Study Quadrupolar Nuclei in Biological Systems (PhD Thesis, Dmitri Ivanov, January 2001).