Faculty Guide

Title

Professor of Biochemistry and Howard Hughes Medical Institute Investigator

Biochemistry
Volen National Center for Complex Systems

Expertise

Dynamics of enzymes. Magnetic resonance methods.

Awards and Honors

Robert Greenberg Lectureship Michigan University (2011)

Charles Slichter Lecture (2010)

Goodman Lecture (2009)

National Lecturer of the Biophysical Society (2009)

Howard Hughes Medical Institute Investigator (2005)

Dayhoff Award from the Biophysical Society (2004)

Alberta Gotthardt and Henry Strage Award for Aspiring Young Science Faculty (2003)

Pfizer Award in Enzyme Chemistry, American Chemical Society (2003)

Young Investigator Award of the International Association for Protein Structure Analysis (2002)

Dorothea Erxleben Prize (1995)

Martin Luther Medal (1995)

Postdoctoral Fellowship from German National Scholarship Foundation and BASF (through 1997) (1995)

Graduate fellowship from the German National Scholarship Foundation (through 1993) (1991)

Captain, 1st Division and National Basketball Teams of Germany (through 1991) (1984 - 1991)

Lessing Medal (1984)

Member, 1st Division and National Basketball Teams of Germany (through 1993) (1981 - 1992)

Courses Taught

BCHM	101a	Advanced Biochemistry: Enzyme Mechanisms
BCHM	102a	Quantitative Approaches to Biochemical Systems
BCHM	103b	Advanced Biochemistry: Information Transfer Mechanisms

Scholarship

Morrison EA, DeKoster GT, Dutta S, Vafabakhsh R, Clarkson MW, Bahl A, Kern D, Ha T, Henzler-Wildman KA.. "Antiparallel EmrE exports drugs by exchanging between asymmetric structures.." Nature 481. (2011): 45-50.

Bosco DA, Eisenmesser EZ, Clarkson MW, Wolf-Watz M, Labeikovsky W, Millet O, Kern D.. "“Dissecting the Microscopic Steps of the Cyclophilin A Enzymatic Cycle on the Biological Substrate HIV Capsid by NMR.”." J. Mol. Biol. 403. 5 (2010): 723-738.

Otten R, Villali J, Kern D, Mulder FA.. "“Probing Microsecond Time Scale Dynamics in Proteins by Methyl (1)H Carr-Purcell-Meiboom-Gill Relaxation Dispersion NMR Measurements. Application to Activation of the Signaling Protein NtrC(r)”.." J Am Chem Soc. 132. 47 (2010): 17004-17014.

Villali, J. and Kern, Dorothee. "“Choreographing an enzyme's dance.”." Curr Opin Chem Biol. 5. 1 (2010): 636-643.

Clarkson MW, Lei M, Eisenmesser EZ, Labeikovsky W, Redfield A. and Kern, Dorothee. "Mesodynamics in the SARS nucleocapsid measured by NMR field cycling." J Biomol NMR 45. 1 (2009): 217-225.

Fraser, J.S., Clarkson, M.W., Degnan, S.C., Erion, R., Kern, D. Alber, T.. "Hidden alternative structures of proline isomerase essential for catalysis." Nature 462. 1 (2009): 669-673.

Gardino, A.K., Villali, J., Kivenson, A., Lei, M., Liu, C.F., Steindel, P. Eisenmesser, E.Z., Labeikovsky, W. Wolf-Watz, M., Clarkson. M.W. and Kern, Dorothee. "Transient non-native bonds promote activation of a signaling protein." Cell 139. 6 (2009): 1109-1118.

Lei, M., Velos, J., Gardino, A., Kivenson, A., Karplus, M. and Kern, Dorothee. "Segmented Transition Pathway of the Signaling Protein Nitrogen Regulatory Protein C." J. Mol. Biol. 392. 3 (2009): 823-836.

Shim S, Yuan JP, Kim JY, Zeng W, Huang G, Milshteyn A, Kern, Dorothee, Muallem S, Ming GL, Worley PF. "Peptidyl-prolyl isomerase FKBP52 controls chemotropic guidance of neuronal growth cones via regulation of TRPC1 channel opening." Neuron 64. 1 (2009): 471-483.

Gardino, A.K. and Kern D.. "¿Functional Dynamics of Response Regulators using NMR Relaxation Techniques¿." Methods in Enzym 423. (2008): :149-165..

Henzler-Wildman KA, Lei M, Thai V, Kerns SJ, Karplus M, Kern D.. "A hierarchy of timescales in protein dynamics is linked to enzyme catalysis." Nature 450. 7171 (2008): 913-916.

Henzler-Wildman KA, Thai V, Lei M, Ott M, Wolf-Watz M, Fenn T, Pozharski E, Wilson MA, Petsko GA, Karplus M, Hübner CG, Kern D. ".¿ Intrinsic motions along an enzymatic reaction trajectory.." Nature 450. 7171 (2008): 838-844.

Henzler-Wildman KA. and Kern D. "Dynamic personalities of proteins." Nature 450. 7172 (2008): 964-972.

Labeikovsky, W., Eisenmeser, E. Z. , Bosco, D.A. and Kern, Dorothee. "¿Structure and dynamics of Pin1 during catalysis by NMR¿." J. Mol. Biol. 367. 5 (2007): 1370-1381.

Thai V, Renesto P, Fowler CA, Brown D, Davis T, Gu W, Pollock DD, Kern D, Raoult D, Eisenmesser EZ. "Structural, Biochemical, and in Vivo Characterization of the First Virally Encoded Cyclophilin from the Mimivirus." J Mol Biol. Aug 29; [Epub ahead of print]. (2007).

Eisenmesser, E.Z., Millet, O., Labeikovsky, W., Korzhnev, D.M., Wolf-Watz, M., Bosco, D.A., Skalicky, J.J., Kay, L.E. and Kern, Dorothee. ""Intrinsic dynamics of an enzyme underlies catalysis"." Nature 438. (2005): 117-121.

Kern, Dorothee Eisenmesser, E.Z. & Wolf-Watz, M.. "Enzyme Dynamics During Catalysis Measured by NMR Spectroscopy." Meth. Enzymol (2005).

Tittmann, K., Neef, H., Golbik, R., Hübner, G. and Kern, Dorothee. "¿Kinetic control of thiamin diphosphate activation in enzymes studied by proton-nitrogen correlated NMR spectroscopy¿." Biochemistry 44. (2005): 8697-8700.

Wemmer, DE. and Kern, Dorothee. ""Beryllofluoride binding mimics phosphorylation of aspartate in response regulators"." J Bacteriol. 187. 24 (2005): 8229-8230.

Bosco, D.A. and Kern, D.. "Catalysis and Binding of Cyclophilin A with Different HIV-1 Capsid Constructs." Biochemistry (2004).

Kern, Dorothee Wolf-Watz, M. Thai, V. Henzler-Wildman, K. Hadjipavlou, G. & Eisenmesser, E.Z.. "Linkage Between Dynamics and Catalysis in a Thermophilic - Mesophilic Enzyme Pair." Nat. Struct. Mol. Biol. (2004).

Gardino, A.K. Volkman, B.F. Cho, H.S. Lee, S.Y. Wemmer, D.E. & Kern, D.. "The NMR Solution Structure of BeF3-Activated Spo0F Reveals the ConformationalSwitch in a Phosphorelay System." J. Mol. Biol. (2003).

Kern, D. and Zuiderweg, E.R.P.. "The Role of Dynamics in Allosteric Regulation." Current Opinion in Structural Biology (2003).