Faculty Guide

Title

Louis and Bessie Rosenfield Professor of Biochemistry

Biochemistry
Volen National Center for Complex Systems

Expertise

Structure-function studies of visual pigments and other cell surface receptors.

Awards and Honors

Louis and Bessie Rosenfield Chair in Biochemistry (1996)

Courses Taught

BCBP	200b	Reading in Macromolecular Structure-Function Analysis
BCHM	100a	Introductory Biochemistry
BCHM	101a	Advanced Biochemistry: Enzyme Mechanisms
BCHM	103b	Advanced Biochemistry: Information Transfer Mechanisms
BCHM	104a	Physical Chemistry of Macromolecules I
CHEM	141a	Thermodynamics and Statistical Thermodynamics

Scholarship

Frederiksen, R., R.K. Crouch, B. Nickle, K.S. Chakrabarti, D. Oprian, Y. Doutalos, N.P. Boyer, and M.C. Cornwall. "Increased Rate of Pigment Regeneration and Dark Adaptation in Rod Photoreceptors Containing 11-cis 4-OH Rhodopsin.." Journal of General Physiology (2012). (forthcoming)

Deupi, X., R. Edwards, A. Singhal, B. Nickle, D. Oprian, G. Schertler, and J. Standfuss. "Structural Basis for Selective Stabilization of the G-Protein-Binding Site in Constitutively Active Rhodopsin.." Proc. Natl. Acad. Sci. U S. A. 109. (2012): 119-24.

Standfuss, J., Edwards, P. C., D’Antona, A., Fransen, M., Xie, G., Oprian, D. D., and Schertler, G. F.. "The structural basis of agonist -induced activation in constitutively active rhodopsin." Nature 471. 7340 (2011): doi:10.1038/nature/09795.

Xie, G., D’Antona, A. M., Edwards, P. C., Fransen, M., Standfuss, J., Schertler, G. F. X., and Oprian, D. D.. "Preparation of an Activated Rhodopsin/Transducin Complex Using a Constitutively Active Mutant of Rhodopsin.." Biochemistry 50. (2011): 10399-407.

Bayburt, T. H., Leitz, A. J., Xie, G., Oprian, D. D.,, and Sligar, S. G.. "Transducin Activation by Isolated Monomeric and Dimeric Rhodopsin in Nanoscale Lipid Bilayers.." submitted (2007).

Standfuss, J., Xie, G., Edwards, P., Li, J., Burghammer, M., Oprian, D. D., and Schertler, G. F. X.. "Crystal Structure of a Thermally Stable Rhodopsin Mutant.." J. Mol. Biol. in press. (2007).

Chen, J., Shi, G., Concepcion, F. A., Xie, G., Oprian, D. D., and Chen, J.. "Stable Rhodopsin/Arrestin Complex Leads to Retinal Degeneration in a Transgenic Mouse Model of Autosomal Dominant Retinitis Pigmentosa.." J. Neurosci. 26. (2006): 11929-37.

Higgins, M. K., Oprian, D. D., and Schertler, G. F.. "Recoverin Binds Exclusively to an amphipathic peptide at the N-Terminus of Rhodopsin Kinase,, Inhibiting Rhodopsin Phosphorylation without Affecting Catalytic Activity of the Kinase.." J. Biol. Chem. 281. (2006): 19426-32.

Tam, B. M., Xie, G., Oprian, D. D., and Moritz, O. L.. "Mislocalized rhodopsin does not require activation to cause retinal degeneration or neurite outgrowth in X. laevis.." J. Neurosci. 26. (2006): 203-9.

Oprian,Daniel, Jin, S. and McKee, T. D.. "An Improved Rhodopsin/EGFP Fusion Protein for Use in the Generation of Transgenic Xenopus laevis." FEBS Lett. 542. (2003): 142-146.

Oprian,Daniel, Jin, S., and Cornwall, M. C.. "Opsin Activation as a Mechanism of Congenital Night Blindness." Nature Neuroscience 6. (2003): 731-735.