Abelson-Bass-Yalem Building, 214
DegreesBrandeis University, Ph.D.
University of California, Berkeley, B.A.
ExpertiseA primary area of interest is macromolecules of rod-like virus particles. These particles form liquid crystals as well as other phases. Because of the simplicity of interparticle interactions a microscopic theory of the phase behavior and bulk properties can be undertaken. We have genetically engineered viruses to produce molecules of new shapes and have calculated how these modifications should lead to the spontaneous formation of structured materials. This is an example of nanobiotechnolgy using a bottoms-up approach of self-assembly. A second area of interest, of direct biological relevance, is exploring the phenomena of "Macromolecular Crowding". Macromolecules occupy 30% of the volume of the cell, strongly influencing inter-molecular interactions. This crowding of molecules causes like species to phase separate into different regions of the cell, leading to macromolecular compartmentalization without the need for any intracellular membrane. A third area of interest is protein crystallization. We are following two approaches. One is the "rational approach" of applying statistical mechanics and thermodynamics to understand the phase behavior of protein solutions using the theory of multicomponent fluids. The second approach is to use microfluidics to develop devices for high throughput screening of crystallization conditions. And finally, we are working on integrating the thermodynamic and high throughput screening approaches.
ProfileThe research group of Seth Fraden is focused on two topics; non-linear chemical dynamics and protein crystallization.
In living systems, chemistry is commonly used in ways that are extremely rare in inanimate systems. For example, muscles consume chemical energy to perform work and oscillating chemical reactions such as the pace maker in the heart control contractions. Working with colleagues in the chemistry department we are using microfluidic manufacturing technology to construct model synthetic systems that contain artificial neural networks coupled to a synthetic musculature.
Protein crystallization is of importance in biotechnology as a necessary step to obtain the structure of proteins. We seek to elucidate the physics of protein crystallization and guided by our understanding of nucleogenesis are developing microfluidic based high throughput crystallization devices. Webpage
|BCBP||297a||Master's Lab Research I|
|BCBP||297b||Master's Lab Research II|
|PHYS||39a||Advanced Physics Laboratory|
Awards and Honors
Innovation Prize of the International Organization of Biological Crystallization (2008)
National Science Foundation Fellowship (1987 - 1989)
Romanowsky, M. B., Heymann, M., Abate, A. R., Krummel, A. T., Fraden, S., Weitz, D. A.. "Functional patterning of PDMS microfluidic devices using integrated chemo-masks." Lab on a Chip 10. 12 (2010): 1521-1524.
Selimovic, S., Gobeaux, F., Fraden, S.. "Mapping and manipulating temperature-concentration phase diagrams using microfluidics." Lab on a Chip 10. (2010): 1696-1699.
Toiya, M., Gonzalez-Ochoa, H. O., Vanag, V. K., Fraden, S., Epstein, I. R.. "Synchronization of Chemical Micro-oscillators." Journal of Physical Chemistry Letters 1. 8 (2010): 1241-1246.
Boukellal, H., Selimovic, S., Jia, Y. W., Cristobal, G., Fraden, S.. "Simple, robust storage of drops and fluids in a microfluidic device." Lab on a Chip 9. 2 (2009): 331-338.
F. Huang, K. Addas, A. Ward, N. T. Flynn, E. Velasco, M. F. Hagan, Z. Dogic, S. Fraden. "Pair Potential of Charged Colloidal Stars." Phys. Rev. Letts. 102. (2009): 108302.
Huang F, Rotstein R, Fraden S, Kasza KE , Flynn NT. "Phase behavior and rheology of attractive rod-like particles." Soft Matter 14. (2009): 2766-2771.
Selimovic, Seila, Jia, Yanwei, Fraden, Seth. "Measuring the Nucleation Rate of Lysozyme using Microfluidics." Crystal Growth & Design 9. (2009): 1806-1810.
Blouwolff, J., Fraden, S.. "Crystallization conditions of membrane protein CLC-ec1: An example outside the crystallization slot." Journal of Crystal Growth 303. 2 (2007): 546-553.
Purdy, K. R., Fraden, S.. "Influence of charge and flexibility on smectic phase formation in filamentous virus suspensions." Physical Review E 76. 1 (2007): Art. No. 011705 .
Shim, J. U., Cristobal, G., Link, D. R., Thorsen, T., Fraden, S.. "Using microfluidics to decouple nucleation and growth of protein crystals." Crystal Growth & Design 7. 11 (2007): 2192-2194.
Shim, J. U., Cristobal, G., Link, D. R., Thorsen, T., Jia, Y. W., Piattelli, K., Fraden, S.. "Control and measurement of the phase behavior of aqueous solutions using microfluidics." Journal of the American Chemical Society 129. 28 (2007): 8825-8835.
Varga, S., Fraden, S.. "Smectic ordering in athermal systems of rodlike triblock copolymers." Journal of Chemical Physics 127. 15 (2007).
Bloustine, J., Virmani, T., Thurston, G. M., Fraden, S.. "Light scattering and phase behavior of lysozyme-poly(ethylene glycol) mixtures." Physical Review Letters 96. 8 (2006).
Blouwolff, J., Fraden, S.. "The coordination number of granular cylinders." Europhysics Letters 76. 6 (2006): 1095-1101.
Dogic, Z., Fraden, S.. "Ordered phases of filamentous viruses." Current Opinion in Colloid & Interface Science 11. 1 (2006): 47-55.
Fraden, S., Dogic, Z.. "Phase Behavior of Rod-Like Viruses and Virus-Sphere Mixtures." Soft Matter, vol. 2: Complex Colloidal Suspensions. vol. 2 Ed. Gompper, G., Schick, M.. Weinheim: Wiley-VCH, 2006
Fraden, Seth Purdy, K.R. Varga, S Galindo, A. & Jackson, G.. "Nematic Phase Transitions in Mixtures of Thin and Thick Colloidal Rods." Phys. Rev. Lett. 94. 057801 (2005).
Purdy, K. R., Varga, S., Galindo, A., Jackson, G., Fraden, S.. "Nematic phase transitions in mixtures of thin and thick colloidal rods." Physical Review Letters 94. 5 (2005): 057801.
Varga, S., Purdy, K., Galindo, A., Fraden, S., Jackson, G.. "Nematic-nematic phase separation in binary mixtures of thick and thin hard rods: Results from Onsager-like theories." Physical Review E 72. 5 (2005).
Dogic, Z., Purdy, K. R., Grelet, E., Adams, M., Fraden, S.. "Isotropic-nematic phase transition in suspensions of filamentous virus and the neutral polymer Dextran." Physical Review E 69. (2004): 051702.
Fraden, Seth & Purdy, K.R.. "Isotropic-Cholesteric Phase Transition of Filamentous Virus Suspensions as a Function of Rod Length and Charge." Phys. Rev. E 70. 061703 (2004).
Fraden,Seth Dogic, Z. Purdy, K. Grelet, E. & Adams, M.. "Isotropic-Nematic Phase Transition in Suspensions of Filamentous Virus and the Neutral Polymer Dextran." Phys. Rev. E 69. 051702 (2004).
Purdy, K. R., Fraden, S.. "Isotropic-cholesteric phase transition of filamentous virus suspensions as a function of rod length and charge." Physical Review E 70. 6 (2004): 061703.
Bloustine, J., Berejnov, V., Fraden, S.. "Measurements of protein-protein interactions by size exclusion chromatography." Biophysical Journal 85. 4 (2003): 2619-2623.
Grelet, E., Fraden, S.. "What is the origin of chirality in the cholesteric phase of virus suspensions?." Physical Review Letters 90. 19 (2003): 198302.
Purdy, K. R., Dogic, Z., Fraden, S., Ruhm, A., Lurio, L., Mochrie, S. G. J.. "Measuring the nematic order of suspensions of colloidal fd virus by x-ray diffraction and optical birefringence." Physical Review E 67. 3 (2003): 031708.
Dogic, Z., Fraden, S.. "Development of model colloidal liquid crystals and the kinetics of the isotropic-smectic transition." Philosophical Transactions of the Royal Society of London Series A-Mathematical Physical and Engineering Sciences 359. 1782 (2001): 997-1014.
Jones, R., Fraden, S., Hamley, I.. "Development of model colloidal liquid crystals and the kinetics of the isotropic-smectic transition - Discussion." Philosophical Transactions of the Royal Society of London Series a-Mathematical Physical and Engineering Sciences 359. 1782 (2001): 1014-1015.
Dassanayake, U., Fraden, S., van Blaaderen, A.. "Structure of electrorheological fluids." Journal of Chemical Physics 112. 8 (2000): 3851-3858.
Dogic, Z., Fraden, S.. "Cholesteric phase in virus suspensions." Langmuir 16. 20 (2000): 7820-7824.
Dogic, Z., Frenkel, D., Fraden, S.. "Enhanced stability of layered phases in parallel hard spherocylinders due to addition of hard spheres." Physical Review E 62. 3 (2000): 3925-3933.
Dogic, Z., Philipse, A. P., Fraden, S., Dhont, J. K. G.. "Concentration-dependent sedimentation of colloidal rods." Journal of Chemical Physics 113. 18 (2000): 8368-8380.
Fraden, S., Kamien, R. D.. "Self-assembly in vivo." Biophysical Journal 78. 5 (2000): 2189-2190.
Adams, M., Dogic, Z., Keller, S. L., Fraden, S.. "Entropically driven microphase transitions in mixtures of colloidal rods and spheres." Nature 393. 6683 (1998): 349-352.
Adams, M., Fraden, S.. "Phase behavior of mixture's of rods (tobacco mosaic virus) and spheres (polyethylene oxide, bovine serum albumin)." Biophysical Journal 74. 1 (1998): 669-677.
Ospeck, M., Fraden, S.. "Solving the Poisson-Boltzmann equation to obtain interaction energies between confined, like-charged cylinders." Journal of Chemical Physics 109. 20 (1998): 9166-9171.
Dogic, Z., Fraden, S.. "Smectic phase in a colloidal suspension of semiflexible virus particles." Physical Review Letters 78. 12 (1997): 2417-2420.
Tang, J. X., Fraden, S.. "Nonmonotonic temperature dependence of the flexibility of bacteriophage fd." Biopolymers 39. 1 (1996): 13-22.
Fraden, S.. "Phase transitions in colloidal suspensions of virus particles." Observation, Prediction, and Simulation of Phase Transitions in Complex Fluids. vol. 460 Ed. Baus, M., Rull, L. F., Ryckaert, J. P.. Kluwer Academic Publishers, 1995. 113 - 164.
Tang, J. X., Fraden, S.. "Isotropic-Cholesteric Phase-Transition in Colloidal Suspensions of Filamentous Bacteriophage-Fd." Liquid Crystals 19. 4 (1995): 459-467.
Hu, Y., Glass, J. L., Griffith, A. E., Fraden, S.. "Observation and Simulation of Electrohydrodynamic Instabilities in Aqueous Colloidal Suspensions." Journal of Chemical Physics 100. 6 (1994): 4674-4682.
Kepler, G. M., Fraden, S.. "Attractive Potential between Confined Colloids at Low Ionic-Strength." Physical Review Letters 73. 2 (1994): 356-359.
Kepler, G. M., Fraden, S.. "Video Microscopy Study of the Potential-Energy of a Colloidal Particle Confined between 2 Plates." Langmuir 10. 8 (1994): 2501-2506.
Ospeck, M., Fraden, S.. "Influence of Reflecting Boundaries and Finite Interfacial Thickness on the Coherent Backscattering Cone." Physical Review E 49. 5 (1994): 4578-4589.
Fraden, S., Maret, G., Caspar, D. L. D.. "Angular-Correlations and the Isotropic-Nematic Phase-Transition in Suspensions of Tobacco Mosaic-Virus." Physical Review E 48. 4 (1993): 2816-2837.
Tang, J. X., Fraden, S.. "Magnetic-Field-Induced Isotropic-Nematic Phase-Transition in a Colloidal Suspension." Physical Review Letters 71. 21 (1993): 3509-3512.
Maier, E. E., Krause, R., Deggelmann, M., Hagenbuchle, M., Weber, R., Fraden, S.. "Liquid-Like Order of Charged Rodlike Particle Solutions." Macromolecules 25. 3 (1992): 1125-1133.
Fraden, S., Maret, G.. "Multiple Light-Scattering from Concentrated, Interacting Suspensions." Physical Review Letters 65. 4 (1990): 512-515.
Fraden, S., Hurd, A. J., Meyer, R. B.. "Electric-Field-Induced Association of Colloidal Particles." Physical Review Letters 63. 21 (1989): 2373-2376.
Fraden, S., Maret, G., Caspar, D. L. D., Meyer, R. B.. "Isotropic-Nematic Phase-Transition and Angular-Correlations in Isotropic Suspensions of Tobacco Mosaic-Virus." Physical Review Letters 63. 19 (1989): 2068-2071.
Srajer, G., Fraden, S., Meyer, R. B.. "Field-Induced Nonequilibrium Periodic Structures in Nematic Liquid-Crystals - Nonlinear Study of the Twist Frederiks Transition." Physical Review A 39. 9 (1989): 4828-4834.
Srajer, G., Fraden, S., Meyer, R. B.. "Measurement of the Viscoelastic Coefficients of Main-Chain Nematic Polymers by an Nmr Technique - Comment." Physical Review Letters 61. 7 (1988): 899-899.
Fraden, S., Meyer, R. B.. "Nonequilibrium Periodic Structures Induced by Rotating and Static Fields in a Lyotropic Nematic Liquid-Crystal - Comment." Physical Review Letters 57. 24 (1986): 3122-3122.
Fraden, S., Hurd, A. J., Meyer, R. B., Cahoon, M., Caspar, D. L. D.. "Magnetic-Field Induced Alignment and Instabilities in Ordered Colloids of Tobacco Mosaic-Virus." Journal De Physique 46. C3 (1985): 85-113.
Hurd, A. J., Fraden, S., Lonberg, F., Meyer, R. B.. "Field-Induced Transient Periodic Structures in Nematic Liquid-Crystals - the Splay Frederiks Transition." Journal De Physique 46. 6 (1985): 905-917.
Meyer, R. B., Lonberg, F., Taratuta, V., Fraden, S., Lee, S. D., Hurd, A. J.. "Measurements of the Anisotropic Viscous and Elastic Properties of Lyotropic Polymer Nematics." Faraday Discussions 79 (1985): 125-132.
Lonberg, F., Fraden, S., Hurd, A. J., Meyer, R. B.. "Periodic Director Patterns in Twist and Splay Frederiks Transitions in Tobacco Mosaic-Virus Liquid-Crystals." Molecular Crystals and Liquid Crystals 106. 3-4 (1984): 419-419.
Lonberg, F., Fraden, S., Hurd, A. J., Meyer, R. E.. "Field-Induced Transient Periodic Structures in Nematic Liquid-Crystals - the Twist-Freedericksz Transition." Physical Review Letters 52. 21 (1984): 1903-1906.
Fraden, S., Caspar, D. L. D., Phillips, W.. "X-Ray-Diffraction Studies of Colloid Crystalline Tmv." Biophysical Journal 37. 2 (1982): A97-A97.