Jeff Gelles

Title

Aron and Imre Tauber Professor of Biochemistry and Molecular Pharmacology

Biochemistry

Expertise

Mechanisms of mechanoenzymes. Stochastic processes in single enzyme molecules. Light microscopy as a tool to study enzyme mechanisms.

Degrees

California Institute of Technology, Ph.D.

Harvard University, B.A.

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Contact

Email:	gelles@brandeis.edu
Phone:	781-736-2377
Office:	Kosow-Wolfson-Rosensweig Building, 208

Webpage

Awards and Honors

HHMI-NIBIB Interfaces Award, "Quantitative Biology: A New Curriculum to Link the Physical and Biomedical Sciences" (2006 - 2008)

National Program Chair, 2006 Annual Meeting, The Biophysical Society (2004)

NIH MERIT Award (2002)

Searle Scholars Award (1990)

Lucille P. Markey Scholar in Biomedical Science (1989 - 1994)

Damon Runyon-Walter Winchell Cancer Fund Postdoctoral Fellowship (1987 - 1988)

National Science Foundation Predoctoral Fellowship (1978 - 1981)

Courses Taught

BCHM	102a	Quantitative Approaches to Biochemical Systems
BCHM	103b	Advanced Biochemistry: Information Transfer Mechanisms
QBIO	120b	Quantitative Biology Instrumentation Laboratory

Scholarship

Smith BA, Daugherty-Clarke K, Goode BL, Gelles J. ".Pathway of actin filament branch formation by Arp2/3 complex revealed by single-molecule imaging.." Proc Natl Acad Sci U S A. 110. 4 (2013): 1285.

Breitsprecher D, Jaiswal R, Bombardier JP, Gould CJ, Gelles J, Goode BL.. "Rocket launcher mechanism of collaborative actin assembly defined by single-molecule imaging.." Science 336. 6085 (2012): 1164-1168.

Friedman, Larry F. and Jeff Gelles. "Mechanism of Transcription Initiation at an Activator-Dependent Promoter Defined by Single-Molecule Observation." Cell 148. 4 (2012): 679-689.

Garcia HG, Sanchez A, Boedicker JQ, Osborne M, Gelles J, Kondev J, Phillips R.. "Operator sequence alters gene expression independently of transcription factor occupancy in bacteria." Cell Rep 2. 1 (2012): 150.

Hoskins, Aaron A., Friedman, Larry J., Gallagher, Sarah S., Crawford, Daniel J., Anderson, Eric G., Wombacher, Richard, Ramirez, Nicholas, Cornish, Virginia W., Gelles, Jeff, Moore, Melissa J.. "Ordered and Dynamic Assembly of Single Spliceosomes." Science 331. 6022 (2011): 1289-1295.

Hoskins, Aaron A., Gelles, Jeff, Moore, Melissa J.. "New insights into the spliceosome by single molecule fluorescence microscopy." Current Opinion in Chemical Biology 15. 6 (2011): 864-870.

Sanchez, Alvaro, Osborne, Melisa L., Friedman, Larry J., Kondev, Jane, Gelles, Jeff. "Mechanism of transcriptional repression at a bacterial promoter by analysis of single molecules." The EMBO Journal 30. (2011): 3940-3946.

Ydenberg, Casey A., Smith, Benjamin A., Breitsprecher, Dennis, Gelles, Jeff, Goode, Bruce L.. "Cease-fire at the leading edge: New perspectives on actin filament branching, debranching, and cross-linking." Cytoskeleton 68. (2011): 598-602.

Douglas S. Martin, Reza Fathi, Timothy J. Mitchison, Jeff Gelles. "FRET measurements of kinesin neck orientation reveal a structural basis for processivity and asymmetry." Proc Natl Acad Sci U S A 107. (2010): 5453-5458.

Meghal Gandhi, Benjamin A. Smith, Miia Bovellan, Ville Paavilainen, Karen Daugherty-Clarke, Jeff Gelles, Pekka Lappalainen, Bruce L. Goode. "GMF is a cofilin homologue that binds Arp2/3 complex to stimulate filament debranching and inhibit actin nucleation." Current Biology 20. (2010): 1-7.

Thoresen, T., Gelles, J.. "Processive movement by a kinesin heterodimer with an inactivating mutation in one head." Biochemistry 47. 36 (2008): 9514-21.

Wong, O. K., Guthold, M., Erie, D. A., Gelles, J.. "Interconvertible lac repressor-DNA loops revealed by single-molecule experiments." PLoS Biol 6. 9 (2008): e232.

Crawford, D. J., Hoskins, A. A., Friedman, L. J., Gelles, J., Moore, M. J.. "Visualizing the splicing of single pre-mRNA molecules in whole cell extract." RNA 14. 1 (2007): 170-9.

Subramanian, R., Gelles, J.. "Two distinct modes of processive kinesin movement in mixtures of ATP and AMP-PNP." J Gen Physiol 130. 5 (2007): 445-55.

Dalal, R. V., Larson, M. H., Neuman, K. C., Gelles, J., Landick, R., Block, S. M.. "Pulling on the Nascent RNA during Transcription Does Not Alter Kinetics of Elongation or Ubiquitous Pausing." Mol Cell 23. 2 (2006): 231-9.

Friedman, L. J., Chung, J., Gelles, J.. "Viewing dynamic assembly of molecular complexes by multi-wavelength single-molecule fluorescence." Biophys J 91. 3 (2006): 1023-31.

Mumm, J. P., Landy, A., Gelles, J.. "Viewing single lambda site-specific recombination events from start to finish." Embo J 25. 19 (2006): 4586-95.

Perkins, T. T., Li, H. W., Dalal, R. V., Gelles, J., Block, S. M.. "Forward and reverse motion of single RecBCD molecules on DNA." Biophys J 86. 3 (2004): 1640-8.

Tolic-Norrelykke, S. F., Engh, A. M., Landick, R., Gelles, J.. "Diversity in the rates of transcript elongation by single RNA polymerase molecules." J Biol Chem 279. 5 (2004): 3292-9.

Yang, W., Gelles, J., Musser, S. M.. "Imaging of single-molecule translocation through nuclear pore complexes." Proc Natl Acad Sci U S A 101. 35 (2004): 12887-92.

Neuman, K. C., Abbondanzieri, E. A., Landick, R., Gelles, J., Block, S. M.. "Ubiquitous transcriptional pausing is independent of RNA polymerase backtracking." Cell 115. 4 (2003): 437-47.

Hua, W., Chung, J., Gelles, J.. "Distinguishing inchworm and hand-over-hand processive kinesin movement by neck rotation measurements." Science 295. 5556 (2002): 844-8.

Dohoney, K. M., Gelles, J.. "Chi-sequence recognition and DNA translocation by single RecBCD helicase/nuclease molecules." Nature 409. 6818 (2001): 370-4.

Yin, H., Artsimovitch, I., Landick, R., Gelles, J.. "Nonequilibrium mechanism of transcription termination from observations of single RNA polymerase molecules." Proc Natl Acad Sci U S A 96. 23 (1999): 13124-9.

Gelles, J., Landick, R.. "RNA polymerase as a molecular motor." Cell 93. 1 (1998): 13-6.

Vugmeyster, Y., Berliner, E., Gelles, J.. "Release of isolated single kinesin molecules from microtubules." Biochemistry 37. 2 (1998): 747-57.

Wang, M. D., Schnitzer, M. J., Yin, H., Landick, R., Gelles, J., Block, S. M.. "Force and velocity measured for single molecules of RNA polymerase." Science 282. 5390 (1998): 902-7.

Young, E. C., Mahtani, H. K., Gelles, J.. "One-headed kinesin derivatives move by a nonprocessive, low-duty ratio mechanism unlike that of two-headed kinesin." Biochemistry 37. 10 (1998): 3467-79.

Hua, W., Young, E. C., Fleming, M. L., Gelles, J.. "Coupling of kinesin steps to ATP hydrolysis." Nature 388. 6640 (1997): 390-3.

Wang, M. D., Yin, H., Landick, R., Gelles, J., Block, S. M.. "Stretching DNA with optical tweezers." Biophys J 72. 3 (1997): 1335-46.

Berliner, E., Young, E. C., Anderson, K., Mahtani, H. K., Gelles, J.. "Failure of a single-headed kinesin to track parallel to microtubule protofilaments." Nature 373. 6516 (1995): 718-21.

Finzi, L., Gelles, J.. "Measurement of lactose repressor-mediated loop formation and breakdown in single DNA molecules." Science 267. 5196 (1995): 378-80.

Gelles, J., Berliner, E., Young, E. C., Mahtani, H. K., Perez-Ramirez, B., Anderson, K.. "Structural and functional features of one- and two-headed biotinated kinesin derivatives." Biophys J 68. 4 Suppl (1995): 276S-281S; discussion 282S.

Gelles, J., Yin, H., Finzi, L., Wong, O. K., Landick, R.. "Single-molecule kinetic studies on DNA transcription and transcriptional regulation." Biophys J 68. 4 Suppl (1995): 73S.

Yin, H., Wang, M. D., Svoboda, K., Landick, R., Block, S. M., Gelles, J.. "Transcription against an applied force." Science 270. 5242 (1995): 1653-7.

Young, E. C., Berliner, E., Mahtani, H. K., Perez-Ramirez, B., Gelles, J.. "Subunit interactions in dimeric kinesin heavy chain derivatives that lack the kinesin rod." J Biol Chem 270. 8 (1995): 3926-31.

Berliner, E., Mahtani, H. K., Karki, S., Chu, L. F., Cronan, J. E., Jr., Gelles, J.. "Microtubule movement by a biotinated kinesin bound to streptavidin-coated surface." J Biol Chem 269. 11 (1994): 8610-5.

Yin, H., Landick, R., Gelles, J.. "Tethered particle motion method for studying transcript elongation by a single RNA polymerase molecule." Biophys J 67. 6 (1994): 2468-78.

Kuo, S. C., Gelles, J., Steuer, E., Sheetz, M. P.. "A model for kinesin movement from nanometer-level movements of kinesin and cytoplasmic dynein and force measurements." J Cell Sci Suppl 14. (1991): 135-8.

Schafer, D. A., Gelles, J., Sheetz, M. P., Landick, R.. "Transcription by single molecules of RNA polymerase observed by light microscopy." Nature 352. 6334 (1991): 444-8.

Gelles, J., Schnapp, B. J., Sheetz, M. P.. "Tracking kinesin-driven movements with nanometre-scale precision." Nature 331. 6155 (1988): 450-3.

Nilsson, T., Gelles, J., Li, P. M., Chan, S. I.. "Chemical modification of the CuA site affects the proton pumping activity of cytochrome c oxidase." Biochemistry 27. 1 (1988): 296-301.

Schnapp, B. J., Gelles, J., Sheetz, M. P.. "Nanometer-scale measurements using video light microscopy." Cell Motil Cytoskeleton 10. 1-2 (1988): 47-53.

Li, P. M., Gelles, J., Chan, S. I., Sullivan, R. J., Scott, R. A.. "Extended X-ray absorption fine structure of copper in CuA-depleted, p-(hydroxymercuri)benzoate-modified, and native cytochrome c oxidase." Biochemistry 26. 8 (1987): 2091-5.

Blair, D. F., Gelles, J., Chan, S. I.. "Redox-linked proton translocation in cytochrome oxidase: the importance of gating electron flow. The effects of slip in a model transducer." Biophys J 50. 4 (1986): 713-33.

Gelles, J., Blair, D. F., Chan, S. I.. "The proton-pumping site of cytochrome c oxidase: a model of its structure and mechanism." Biochim Biophys Acta 853. 3-4 (1986): 205-36.

Gelles, J., Chan, S. I.. "Chemical modification of the CuA center in cytochrome c oxidase by sodium p-(hydroxymercuri)benzoate." Biochemistry 24. 15 (1985): 3963-72.

Cantley, L. C., Jr., Gelles, J., Josephson, L.. "Reaction of (Na-K)ATPase with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole: evidence for an essential tyrosine at the active site." Biochemistry 17. 3 (1978): 418-25.

Faculty Guide

Jeff Gelles

Smith BA, Daugherty-Clarke K, Goode BL, Gelles J. ".Pathway of actin filament branch formation by Arp2/3 complex revealed by single-molecule imaging.." Proc Natl Acad Sci U S A. 110. 4 (2013): 1285.

Breitsprecher D, Jaiswal R, Bombardier JP, Gould CJ, Gelles J, Goode BL.. "Rocket launcher mechanism of collaborative actin assembly defined by single-molecule imaging.." Science 336. 6085 (2012): 1164-1168.

Friedman, Larry F. and Jeff Gelles. "Mechanism of Transcription Initiation at an Activator-Dependent Promoter Defined by Single-Molecule Observation." Cell 148. 4 (2012): 679-689.

Garcia HG, Sanchez A, Boedicker JQ, Osborne M, Gelles J, Kondev J, Phillips R.. "Operator sequence alters gene expression independently of transcription factor occupancy in bacteria." Cell Rep 2. 1 (2012): 150.

Hoskins, Aaron A., Friedman, Larry J., Gallagher, Sarah S., Crawford, Daniel J., Anderson, Eric G., Wombacher, Richard, Ramirez, Nicholas, Cornish, Virginia W., Gelles, Jeff, Moore, Melissa J.. "Ordered and Dynamic Assembly of Single Spliceosomes." Science 331. 6022 (2011): 1289-1295.

Hoskins, Aaron A., Gelles, Jeff, Moore, Melissa J.. "New insights into the spliceosome by single molecule fluorescence microscopy." Current Opinion in Chemical Biology 15. 6 (2011): 864-870.

Sanchez, Alvaro, Osborne, Melisa L., Friedman, Larry J., Kondev, Jane, Gelles, Jeff. "Mechanism of transcriptional repression at a bacterial promoter by analysis of single molecules." The EMBO Journal 30. (2011): 3940-3946.

Ydenberg, Casey A., Smith, Benjamin A., Breitsprecher, Dennis, Gelles, Jeff, Goode, Bruce L.. "Cease-fire at the leading edge: New perspectives on actin filament branching, debranching, and cross-linking." Cytoskeleton 68. (2011): 598-602.

Douglas S. Martin, Reza Fathi, Timothy J. Mitchison, Jeff Gelles. "FRET measurements of kinesin neck orientation reveal a structural basis for processivity and asymmetry." Proc Natl Acad Sci U S A 107. (2010): 5453-5458.

Meghal Gandhi, Benjamin A. Smith, Miia Bovellan, Ville Paavilainen, Karen Daugherty-Clarke, Jeff Gelles, Pekka Lappalainen, Bruce L. Goode. "GMF is a cofilin homologue that binds Arp2/3 complex to stimulate filament debranching and inhibit actin nucleation." Current Biology 20. (2010): 1-7.

Thoresen, T., Gelles, J.. "Processive movement by a kinesin heterodimer with an inactivating mutation in one head." Biochemistry 47. 36 (2008): 9514-21.

Wong, O. K., Guthold, M., Erie, D. A., Gelles, J.. "Interconvertible lac repressor-DNA loops revealed by single-molecule experiments." PLoS Biol 6. 9 (2008): e232.

Crawford, D. J., Hoskins, A. A., Friedman, L. J., Gelles, J., Moore, M. J.. "Visualizing the splicing of single pre-mRNA molecules in whole cell extract." RNA 14. 1 (2007): 170-9.

Subramanian, R., Gelles, J.. "Two distinct modes of processive kinesin movement in mixtures of ATP and AMP-PNP." J Gen Physiol 130. 5 (2007): 445-55.

Dalal, R. V., Larson, M. H., Neuman, K. C., Gelles, J., Landick, R., Block, S. M.. "Pulling on the Nascent RNA during Transcription Does Not Alter Kinetics of Elongation or Ubiquitous Pausing." Mol Cell 23. 2 (2006): 231-9.

Friedman, L. J., Chung, J., Gelles, J.. "Viewing dynamic assembly of molecular complexes by multi-wavelength single-molecule fluorescence." Biophys J 91. 3 (2006): 1023-31.

Mumm, J. P., Landy, A., Gelles, J.. "Viewing single lambda site-specific recombination events from start to finish." Embo J 25. 19 (2006): 4586-95.

Perkins, T. T., Li, H. W., Dalal, R. V., Gelles, J., Block, S. M.. "Forward and reverse motion of single RecBCD molecules on DNA." Biophys J 86. 3 (2004): 1640-8.

Perkins, T. T., Li, H. W., Dalal, R. V., Gelles, J., Block, S. M.. "Forward and reverse motion of single RecBCD molecules on DNA." Biophys J 86. 3 (2004): 1640-8.

Tolic-Norrelykke, S. F., Engh, A. M., Landick, R., Gelles, J.. "Diversity in the rates of transcript elongation by single RNA polymerase molecules." J Biol Chem 279. 5 (2004): 3292-9.

Tolic-Norrelykke, S. F., Engh, A. M., Landick, R., Gelles, J.. "Diversity in the rates of transcript elongation by single RNA polymerase molecules." J Biol Chem 279. 5 (2004): 3292-9.

Yang, W., Gelles, J., Musser, S. M.. "Imaging of single-molecule translocation through nuclear pore complexes." Proc Natl Acad Sci U S A 101. 35 (2004): 12887-92.

Yang, W., Gelles, J., Musser, S. M.. "Imaging of single-molecule translocation through nuclear pore complexes." Proc Natl Acad Sci U S A 101. 35 (2004): 12887-92.

Neuman, K. C., Abbondanzieri, E. A., Landick, R., Gelles, J., Block, S. M.. "Ubiquitous transcriptional pausing is independent of RNA polymerase backtracking." Cell 115. 4 (2003): 437-47.

Hua, W., Chung, J., Gelles, J.. "Distinguishing inchworm and hand-over-hand processive kinesin movement by neck rotation measurements." Science 295. 5556 (2002): 844-8.

Dohoney, K. M., Gelles, J.. "Chi-sequence recognition and DNA translocation by single RecBCD helicase/nuclease molecules." Nature 409. 6818 (2001): 370-4.

Yin, H., Artsimovitch, I., Landick, R., Gelles, J.. "Nonequilibrium mechanism of transcription termination from observations of single RNA polymerase molecules." Proc Natl Acad Sci U S A 96. 23 (1999): 13124-9.

Gelles, J., Landick, R.. "RNA polymerase as a molecular motor." Cell 93. 1 (1998): 13-6.

Vugmeyster, Y., Berliner, E., Gelles, J.. "Release of isolated single kinesin molecules from microtubules." Biochemistry 37. 2 (1998): 747-57.

Wang, M. D., Schnitzer, M. J., Yin, H., Landick, R., Gelles, J., Block, S. M.. "Force and velocity measured for single molecules of RNA polymerase." Science 282. 5390 (1998): 902-7.

Young, E. C., Mahtani, H. K., Gelles, J.. "One-headed kinesin derivatives move by a nonprocessive, low-duty ratio mechanism unlike that of two-headed kinesin." Biochemistry 37. 10 (1998): 3467-79.

Hua, W., Young, E. C., Fleming, M. L., Gelles, J.. "Coupling of kinesin steps to ATP hydrolysis." Nature 388. 6640 (1997): 390-3.

Wang, M. D., Yin, H., Landick, R., Gelles, J., Block, S. M.. "Stretching DNA with optical tweezers." Biophys J 72. 3 (1997): 1335-46.

Berliner, E., Young, E. C., Anderson, K., Mahtani, H. K., Gelles, J.. "Failure of a single-headed kinesin to track parallel to microtubule protofilaments." Nature 373. 6516 (1995): 718-21.

Finzi, L., Gelles, J.. "Measurement of lactose repressor-mediated loop formation and breakdown in single DNA molecules." Science 267. 5196 (1995): 378-80.

Gelles, J., Berliner, E., Young, E. C., Mahtani, H. K., Perez-Ramirez, B., Anderson, K.. "Structural and functional features of one- and two-headed biotinated kinesin derivatives." Biophys J 68. 4 Suppl (1995): 276S-281S; discussion 282S.

Gelles, J., Yin, H., Finzi, L., Wong, O. K., Landick, R.. "Single-molecule kinetic studies on DNA transcription and transcriptional regulation." Biophys J 68. 4 Suppl (1995): 73S.

Yin, H., Wang, M. D., Svoboda, K., Landick, R., Block, S. M., Gelles, J.. "Transcription against an applied force." Science 270. 5242 (1995): 1653-7.

Young, E. C., Berliner, E., Mahtani, H. K., Perez-Ramirez, B., Gelles, J.. "Subunit interactions in dimeric kinesin heavy chain derivatives that lack the kinesin rod." J Biol Chem 270. 8 (1995): 3926-31.

Berliner, E., Mahtani, H. K., Karki, S., Chu, L. F., Cronan, J. E., Jr., Gelles, J.. "Microtubule movement by a biotinated kinesin bound to streptavidin-coated surface." J Biol Chem 269. 11 (1994): 8610-5.

Yin, H., Landick, R., Gelles, J.. "Tethered particle motion method for studying transcript elongation by a single RNA polymerase molecule." Biophys J 67. 6 (1994): 2468-78.

Kuo, S. C., Gelles, J., Steuer, E., Sheetz, M. P.. "A model for kinesin movement from nanometer-level movements of kinesin and cytoplasmic dynein and force measurements." J Cell Sci Suppl 14. (1991): 135-8.

Schafer, D. A., Gelles, J., Sheetz, M. P., Landick, R.. "Transcription by single molecules of RNA polymerase observed by light microscopy." Nature 352. 6334 (1991): 444-8.

Gelles, J., Schnapp, B. J., Sheetz, M. P.. "Tracking kinesin-driven movements with nanometre-scale precision." Nature 331. 6155 (1988): 450-3.

Nilsson, T., Gelles, J., Li, P. M., Chan, S. I.. "Chemical modification of the CuA site affects the proton pumping activity of cytochrome c oxidase." Biochemistry 27. 1 (1988): 296-301.

Schnapp, B. J., Gelles, J., Sheetz, M. P.. "Nanometer-scale measurements using video light microscopy." Cell Motil Cytoskeleton 10. 1-2 (1988): 47-53.

Li, P. M., Gelles, J., Chan, S. I., Sullivan, R. J., Scott, R. A.. "Extended X-ray absorption fine structure of copper in CuA-depleted, p-(hydroxymercuri)benzoate-modified, and native cytochrome c oxidase." Biochemistry 26. 8 (1987): 2091-5.

Blair, D. F., Gelles, J., Chan, S. I.. "Redox-linked proton translocation in cytochrome oxidase: the importance of gating electron flow. The effects of slip in a model transducer." Biophys J 50. 4 (1986): 713-33.

Gelles, J., Blair, D. F., Chan, S. I.. "The proton-pumping site of cytochrome c oxidase: a model of its structure and mechanism." Biochim Biophys Acta 853. 3-4 (1986): 205-36.

Gelles, J., Chan, S. I.. "Chemical modification of the CuA center in cytochrome c oxidase by sodium p-(hydroxymercuri)benzoate." Biochemistry 24. 15 (1985): 3963-72.

Cantley, L. C., Jr., Gelles, J., Josephson, L.. "Reaction of (Na-K)ATPase with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole: evidence for an essential tyrosine at the active site." Biochemistry 17. 3 (1978): 418-25.

Gelles, J., Klapper, M. H.. "Pseudo-dynamic contact surface areas: estimation of apolar bonding." Biochim Biophys Acta 533. 2 (1978): 465-77.