About LATE-PCR
- Pierce, K.E. and Wangh, L.J. (2007) "LATE-PCR and allied technologies: Real-time detection strategies for rapid, reliable diagnosis from single cells." In: Single Cell Diagnostics, Methods in Molecular Medicine Series (ed. Alan Thornhill), Series Editor John Walker, Humana Press, UK.
- Sanchez, J. A., Abramowitz, J.D., Salk, J.J., Reis, Jr, A.H., Rice, J.E., Pierce, K.E., and Wangh, L.J. (2006) Two-temperature LATE-PCR endpoint genotyping, BMC Biotechnology, 6:44.
- Salk, J.J., Sanchez, J.A., Pierce, K.E., Rice, J.E., Soares, K.C., and Wangh, L.J. (2006) Direct amplification of single-stranded DNA for pyrosequencing using Linear-After-The-Exponential (LATE)-PCR, Analytical Biochemistry, 353:124-132.
- Pierce, K.E., Sanchez, J.A., Rice, J.E., and Wangh, L.J. (2005) Linear-After-The-Exponential (LATE)-PCR: Primer design criteria for high yields of specific single-stranded DNA and improved real-time detection, Proc Natl Acad Sci USA, 102:8609-8614.
- Sanchez, J.A., Pierce, K.E., Rice, J.E., and Wangh, L.J. (2004) Linear-After-The-Exponential (LATE)-PCR: An advanced method of asymmetric PCR and its uses in quantitative real-time analysis, Proc Natl Acad Sci USA, 101(7):1933-1938.
- Pierce, K.E., Rice, J.E., Sanchez, J.A., and Wangh, L.J. (2003) Detection of cystic fibrosis alleles from single cells using molecular beacons and a novel method of asymmetric real-time PCR, Molecular Human Reproduction, 9(12):815-820.
- Rice, J.E., Sanchesz, J.A., Pierce, K.E., Reis, A.H., Osborne, A., and Wangh, L.J. (2007) Monoplex/multiplex linear-after-the-exponential-PCR assays combined with PrimeSafe and Dilute-'N'-Go sequencing, Nature Protocols, 2(10): 2429-2438.
About RT-LATE-PCR
- Hartshorn C, Wangh LJ. One-step RT-LATE-PCR for mRNA and viral RNA detection and quantification. RT-PCR Protocols: Second Edition, Methods in Molecular Biology series, The Humana Press Inc., In press
- Hartshorn, C., Eckert, J.J., Hartung, O., and Wangh, L.J. (2007) Single-cell duplex RT-LATE-PCR reveals Oct4 and Xist RNA gradients in 8-cell embryos, BMC Biotechnology, 7:87.
About PurAmp and its uses
- Hartshorn, C., Anshelevich, A., Jia, Y., and Wangh, L.J. (2007) Early Onset of Heat-Shock Response in Mouse Embryos Revealed by Quantification of Stress-Inducible hsp70i RNA, Gene Regulation and Systems Biolgy, 1: 1-9.
- Hartshorn, C., Anshelevich, A., and Wangh, L.J. (2005) Laser zona-drilling does not induce hsp70i transcription in blastomeres of 8-cell mouse embryos, Fertility and Sterility, 84(5):1547-1550.
- Hartshorn, C., Anshelevich, A., and Wangh, L.J. (2005) Rapid, single-tube method for quantitative preparation and analysis of RNA and DNA in samples as small as one cell, BMC Biotechnology, 5:2.
About mouse embryos
- Hartshorn, C., Rice, J.E., and Wangh, L.J. (2004) Optimized real-time RT-PCR for quantitative measurements of DNA and RNA in single embryos and blastomeres, A-Z of Quantitative PCR, IUL Biotechnology Series, 5 (ed. S.A. Bustin), La Jolla: International University Line; 675-702.
- Hartshorn, C., Rice, J.E., and Wangh, L.J. (2003) Differential pattern of Xist RNA accumulation in single blastomeres isolated from 8-cell stage mouse embryos following laser zona drilling, Molecular Reproduction and Development, 64:41-51.
- Hartshorn, C., Rice, J.E., and Wangh, L.J. (2002) Developmentally-regulated changes of Xist RNA levels in single preimplantation mouse embryos, as revealed by quantitative real-time PCR, Molecular Reproduction and Development; 61:425-436.
Mitochondrial Diagnostics
- Osborne, A., Reis, A.H., Bach, L., Wangh, L. (2009) Single-Molecule LATE-PCR Analysis of Human Mitochondrial Genomic Sequence Variations, PLoS ONE 4(5).
About single cell genomics using conventional PCR
- Pierce, K.E., and Wangh, L.J. (2004) Effectiveness and limitations of uracil-DNA glycosylases in sensitive real-time PCR assays, BioTechniques 36(1): 44-48.
- Rice, J.E., Sanchez, J.A., Pierce, K.E., and Wangh, L.J. (2002) Real-time PCR with molecular beacons provides a highly accurate assay for detection of Tay-Sachs alleles in single cells, Prenatal Diagnosis, 22:1130-1134.
- Pierce, K., Rice, J.E., Sanchez, J.A., and Wangh, L.J. (2002) QuantiLyse: Reliable DNA amplification from single cells, BioTechniques 32(5):1106-1111.
- Pierce, K.E., Rice, J.E., Sanchez, J.A., Brenner, C., and Wangh, L.J. (2000) Real-time PCR using molecular beacons for accurate detection of the Y chromosome in single human blastomeres, Molecular Human Genetics, 6(12):1155-1164.
- Pierce, K.E., and Wangh, L.J. Linear-After-The-Exponential Polymerase Chain Reaction and Allied Technologies, Methods in Molecular Medicine: Single Cell Diagnostics: Methods and Protocols 7:65-85.
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