People


Contact Expertise / Current Work
larry wangh

Lawrence Wangh

Professor of Biology

Bassine 103
(781) 736-3110
wangh@brandeis.edu

Professor Larry Wangh '68 and his lab focus on the development of diagnostic assays using the patented Linear-After-The-Exponential (LATE)-PCR technique of DNA amplification.
kenneth pierce

Kenneth Pierce

Senior Research Scientist

Bassine 127
(781) 736-3111
pierce@brandeis.edu

I am a co-inventor of LATE-PCR and other molecular detection technologies. My expertise includes the detection of RNA viruses and antibiotic resistance genes in bacteria. I am currently working on a detection test for 14 bacterial species associated with periodontal disease. Each species' target sequence is amplified using a specific limiting primer and detected using a hybridization probe. Multiple species can be identified using different probes in the same color by using different melting temperatures and testing over a wide range of temperatures.
john rice

John Rice

Senior Research Scientist

Bassine 127
(781) 736-3111
rice@brandeis.edu

My general area of research involves assay development for human infectious diseases. In past two years I have developed three different nucleic acid tests (NATs) for drug resistance in tuberculosis. Each assay involves the use of our patented technologies (LATE-PCR, Lights-On/Lights-Off Probes) to uniquely identify the genetic changes that are the causes of drug resistance. In addition, I have developed a NAT that can detect a single mutation across an entire gene in a single tube. This allows for screening of samples that now can only be done by direct sequencing.
j. aquiles sanchez

J. Aquiles Sanchez

Senior Research Scientist

Bassine 127
(781) 736-3111
sanchez@brandeis.edu

I have a long-standing interest in developing LATE-PCR assays for cancer diagnostics and enhancing nucleic acid amplification reactions. I am currently developing a highly multiplex, single-tube LATE-PCR assay for simultaneous genotyping of 13 high-risk human papillomavirus types linked to cervical cancers. Areas of expertise include PCR amplification, primer/assay design, mutation discovery & SNP genotyping, molecular beacons & linear oligonucleotide probes design, melting curve analysis, nucleic acid sample preparation, DNA sequence analysis.
Nicky

Nicky Sirianni

Research Scientist

Bassine 127
(781) 736-3111
nsirianni@brandeis.edu

I have my M.S. degree from the University of Pittsburgh School of Medicine in Molecular Genetics and Biochemistry. My projects include designing a multi-plex STI detection assay for HAIN Lifescience. I am also exploring Expo-Linear PCR methods for identification of mosquitos. When I am not in the lab, I enjoy baking and coaching my son's soccer team.
adam osborne

Adam Osborne

Research Scientist

Bassine 127
(781) 736-3111
aosborne@brandeis.edu

I earned my B.A. in Biochemistry and Molecular Biology from the College of Wooster, studying biological control of plant pathogens. I then earned my Ph.D. from Brandies University studying mitochondrial mutational load using LATE-PCR. I am currently a staff scientist in the Wangh Laboratory studying mitochondria’s role in disease development. I am also interested in plant pathology, its control and detection.
alex

Alexandra Over

Research Technician

Bassine 127
(781) 736-3111
alexover13@gmail.com

After graduating Brandeis in 2013, I became interested in molecular diagnostics while volunteering at the Philippines' National Center for Pulmonary Research. Witnessing PCR's clinical significance continues to motivate my current research on additive reagents that improve PCR sensitivity and reliability. My work optimizing these reagents is a continuation of over 15 years of development in the Wangh laboratory and includes investigation of their function as reversible hot-starts and SNP assay enhancers.
kim

Kimberly Montano

Undergraduate

Bassine 127
(781) 736-3111
kmontano@brandeis.edu

I am a rising senior at Brandeis, and with Dr. Aquiles Sanchez, helped build a LATE-PCR HPV assay for identification of 14 High-Risk HPV types correlated with cervical cancer. My goal is to identify combinations of High-Risk HPV types using the HPV LATE-PCR assay and mixtures of synthetic (non-viral) targets.
jane

Huijun (Jane) Yuan

Lab Administrator &

Research Technician

Bassine 103
(781) 736-3111
huijun@brandeis.edu

I am a 2015 Brandeis MS Biotechnology graduate, and has been working in Wangh Laboratory since 2015. Responsibilities include general management, grant administration, maintenance of laboratory records and website updating. I am also working on a research project about tick and tick-born diseases tests with LATE-PCR technology.