Publications


About LATE-PCR

  1. Pierce KE and Wangh LJ (2015). Low-concentration initiator primers improve the amplification of gene targets with high sequence variability. Methods Mol Biol. 1275:73-89. > See PubMed
  2. Tetrault SM, Rice JE, Wangh LJ, Sanchez JA (2014). Single-Tube Mutation Scanning of The Epidermal Growth Factor Receptor Gene Using Multiplex LATE-PCR and Lights-On/Lights-Off Probes. J Mol Biomark Diagn. May 1;5(3):1000175. > See PDF
  3. Jia Y, Sanchez JA, Wangh LJ (2014). Kinetic hairpin oligonucleotide blockers for selective amplification of rare mutations. Sci Rep. 4:5921. > See PDF
  4. Jia Y, Mak PI, Massey C, Martins RP, Wangh LJ (2013). Construction of a microfluidic chip, using dried-down reagents, for LATE-PCR amplification and detection of single-stranded DNA. Lab Chip. 13(23):4635-41. > See PDF
  5. Rice LM, Reis AH Jr, Mistry R, Khan H, Khosla P, Bharya S, Wangh LJ (2013). Design and construction of a single tube, quantitative endpoint, LATE-PCR multiplex assay for ventilator-associated pneumonia. J Appl Microbiol. 115(3):818-27. See PubMed
  6. Pierce KE, Peter H, Bachman TT, Volpe C, Mistry R, Rice JE, and Wangh LJ (2012). Rapid Detection of TEM-type Extended Spectrum β-Lactamase (ESBL) mutations using multiple Lights-On-Lights-Off Probes with single-stranded DNA amplification. J Mol Diagn. 15(3):291-8. > See PDF
  7. Gentile NL, Dillier AM, Williams GV, Ackers J, Reis AH Jr, Rice LM, Wangh LJ, Czajka JW, Kost GJ (2013). Verification of monoplex and multiplex linear-after-the-exponential PCR gene-specific sepsis assays using clinical isolates. J Appl Microbiol. 114(2):586-94. > See PDF
  8. Rice LM, Reis AH Jr, Ronish B, Carver-Brown RK, Czajka JW, Gentile N, Kost G, Wangh LJ (2013). Design of a single-tube, endpoint, linear-after-the-exponential-PCR assay for 17 pathogens associated with sepsis. J Appl Microbiol. 114(2):457-69. > See PDF
  9. Carver-Brown RK, Reis AH Jr, Rice LM, Czajka JW, Wangh LJ (2012). Design and Construction of a Single-Tube, LATE-PCR, Multiplex Endpoint Assay with Lights-On/Lights-Off Probes for the Detection of Pathogens Associated with Sepsis. J Pathog. 2012:424808. > See PDF
  10. Pierce KE, Khan H, Mistry R, Goldenberg SD, French GL, and Wangh LJ (2012). Rapid detection of sequence variation in Clostridium difficile genes using LATE-PCR with multiple mismatch-tolerant hybridization probes. J Microbiol Methods. 91(2):269-75. > See PDF
  11. Rice JE, Reis AH Jr, Rice LM, Carver-Brown RK, Wangh LJ (2012). Fluorescent signatures for variable DNA sequences. Nucleic Acids Res. 40(21):e164. > See PDF
  12. Jia Y, Hartshorn C, Hartung O, Wangh LJ (2010). Heat shock memory in preimplantation mouse embryos. Fertil Steril. 93(8):2760-3. > See PDF
  13. Jia Y, Osborne A, Rice JE, Wangh LJ (2010). Dilute-'N'-Go dideoxy sequencing of all DNA strands generated in multiplex LATE-PCR assays. Nucleic Acids Res. 38(11):e119. > See PDF
  14. Rice, J.E., Sanchez, J.A., Pierce, K.E., Reis, A.H., Osborne, A., and Wangh, L.J. (2007). Monoplex/multiplex linear-after-the-exponential-PCR assays combined with PrimeSafe and Dilute-'N'-Go sequencing, Nature Protocols. 2(10):2429-2438. > See PDF
  15. Pierce, K.E. and Wangh, L.J. (2007). "LATE-PCR and allied technologies: Real-time detection strategies for rapid, reliable diagnosis from single cells." In: Single Cell Diagnostics, Methods in Molecular Medicine Series (ed. Alan Thornhill), Series Editor John Walker, Humana Press, UK. > See PDF
  16. Sanchez, J. A., Abramowitz, J.D., Salk, J.J., Reis, Jr, A.H., Rice, J.E., Pierce, K.E., and Wangh, L.J. (2006) Two-temperature LATE-PCR endpoint genotyping, BMC Biotechnology, 6:44. > See PDF
  17. Salk, J.J., Sanchez, J.A., Pierce, K.E., Rice, J.E., Soares, K.C., and Wangh, L.J. (2006) Direct amplification of single-stranded DNA for pyrosequencing using Linear-After-The-Exponential (LATE)-PCR, Analytical Biochemistry, 353:124-132. > See PDF
  18. Pierce, K.E., Sanchez, J.A., Rice, J.E., and Wangh, L.J. (2005) Linear-After-The-Exponential (LATE)-PCR: Primer design criteria for high yields of specific single-stranded DNA and improved real-time detection, Proc Natl Acad Sci USA, 102:8609-8614. > See PDF
  19. Sanchez, J.A., Pierce, K.E., Rice, J.E., and Wangh, L.J. (2004) Linear-After-The-Exponential (LATE)-PCR: An advanced method of asymmetric PCR and its uses in quantitative real-time analysis, Proc Natl Acad Sci USA, 101(7):1933-1938. > See PDF
  20. Pierce, K.E., Rice, J.E., Sanchez, J.A., and Wangh, L.J. (2003) Detection of cystic fibrosis alleles from single cells using molecular beacons and a novel method of asymmetric real-time PCR, Molecular Human Reproduction, 9(12):815-820. > See PDF

 About RT-LATE-PCR

  1. Pierce KE, Wangh LJ (2013). Rapid detection and identification of hepatitis C virus (HCV) sequences using mismatch-tolerant hybridization probes: A general method for analysis of sequence variation. Biotechniques. 55(3):125-32. > See PDF
  2. Ronish B, Hakhverdyan M, Ståhl K, Gallardo C, Fernandez-Pinero J, Belák S, Leblanc N, Wangh L. (2011) Design and verification of a highly reliable Linear-After-The-Exponential PCR (LATE-PCR) assay for the detection of African swine fever virus. J Virol Methods. 172(1-2):8-15. > See PDF
  3. K.E. Pierce, R. Mistry, S.M. Reid, S. Bharya, J.P. Dukes, C. Hartshorn, D.P. King and L.J. Wangh (2010).  Design and optimization of a novel reverse transcription linear-after-the-exponential PCR for the detection of foot-and-mouth disease virus, Journal of Applied Microbiology. > See PDF
  4. Reid SM, Pierce KE, Mistry R, Bharya S, Dukes JP, Volpe C, Wangh LJ, King DP (2010). Pan-serotypic detection of foot-and-mouth disease virus by RT linear-after-the-exponential PCR. Mol Cell Probes. 24(5):250-5. > See PDF
  5. Hartshorn C, Wangh LJ (2010) One-step RT-LATE-PCR for mRNA and viral RNA detection and quantification. RT-PCR Protocols: Second Edition, Methods in Molecular Biology, Methods Mol Biol, 630:153-85. > See PubMed
  6. Pierce, K.E., and Wangh, L.J. (2007) Linear-After-The-Exponential Polymerase Chain Reaction and Allied Technologies. Real-time detection strategies for rapid, reliable diagnosis from single cells. Methods in Molecular Medicine: Single Cell Diagnostics: Methods and Protocols 7:65-85. > See PDF
  7. Hartshorn, C., Eckert, J.J., Hartung, O., and Wangh, L.J. (2007) Single-cell duplex RT-LATE-PCR reveals Oct4 and Xist RNA gradients in 8-cell embryos, BMC Biotechnology, 7:87. > See PDF
  8. Hartshorn, C., Rice, J.E., and Wangh, L.J. (2004) Optimized real-time RT-PCR for quantitative measurements of DNA and RNA in single embryos and blastomeresA-Z of Quantitative PCR, IUL Biotechnology Series, 5 (ed. S.A. Bustin), La Jolla: International University Line; 675-702. > See PDF
  9. Pierce, K.E., and Wangh, L.J. (2004) Effectiveness and limitations of uracil-DNA glycosylases in sensitive real-time PCR assays, BioTechniques 36(1): 44-48. > See PDF
  10. Hartshorn, C., Rice, J.E., and Wangh, L.J. (2003) Differential pattern of Xist RNA accumulation in single blastomeres isolated from 8-cell stage mouse embryos following laser zona drilling,  Molecular Reproduction and Development, 64:41-51. > See PDF
  11. Hartshorn, C., Rice, J.E., and Wangh, L.J. (2002) Developmentally-regulated changes of Xist RNA levels in single preimplantation mouse embryos, as revealed by quantitative real-time PCR,  Molecular Reproduction and Development; 61:425-436. > See PDF
  12. Rice, J.E., Sanchez, J.A., Pierce, K.E., and Wangh, L.J. (2002) Real-time PCR with molecular beacons provides a highly accurate assay for detection of Tay-Sachs alleles in single cells, Prenatal Diagnosis, 22:1130-1134. > See PDF
  13. Pierce, K., Rice, J.E., Sanchez, J.A., and Wangh, L.J. (2002) QuantiLyse: Reliable DNA amplification from single cells, BioTechniques 32(5):1106-1111. > See PDF
  14. Pierce, K.E., Rice, J.E., Sanchez, J.A., Brenner, C., and Wangh, L.J. (2000) Real-time PCR using molecular beacons for accurate detection of the Y chromosome in single human blastomeres, Molecular Human Genetics, 6(12):1155-1164. > See PDF

About PurAmp and Its Uses

  1. Hartshorn, C., Anshelevich, A., Jia, Y., and Wangh, L.J. (2007) Early Onset of Heat-Shock Response in Mouse Embryos Revealed by Quantification of Stress-Inducible hsp70i RNA, Gene Regulation and Systems Biolgy 2007:1, 365-373. > See PDF
  2. Hartshorn, C., Anshelevich, A., and Wangh, L.J. (2005) Laser zona-drilling does not induce hsp70i transcription in blastomeres of 8-cell mouse embryos, Fertility and Sterility, 84(5):1547-1550. > See PDF
  3. Hartshorn, C., Anshelevich, A., and Wangh, L.J. (2005) Rapid, single-tube method for quantitative preparation and analysis of RNA and DNA in samples as small as one cell, BMC Biotechnology, 5:2. > See PDF

About Mitochondrial Diagnostics

  1. Rice LM, Reis AH Jr, Wangh LJ (2014) Virtual Barcoding using LATE-PCR and Lights-On/Lights-Off probes: identification of nematode species in a closed-tube reaction. Mitochondrial DNA A DNA MappSeq Anal, 27(2):1358-63. See PDF
  2. Osborne, A., Reis, A.H., Bach, L., Wangh, L. (2009) Single-Molecule LATE-PCR Analysis of Human Mitochondrial Genomic Sequence Variations, PLoS ONE 4(5). > See PDF