Maria-Eirini Pandelia
Degrees
Technical University of Berlin, Ph.D.National Technical University of Athens, M.S.
University of Patras, B.S.
Expertise
My research relies on the synteny of bioinformatic, spectroscopic, structural and kinetic methods to identify and establish the modus operandii of metal-containing enzymes and delineate their catalytic and functional repertoire. My main experise is in EPR and Moessbauer spectroscopy and the application of those techniques for the characterization of the electronic and geometric structure of (bio)inorganic complexes.Profile
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My work is centered on delineating the mechanisms according to which metalloproteins involved in processes essential for life perform the activation of small (or larger) molecules, how the specific identity of the metals in the active sites allows their chemical diversion and selectivity and what the functional role of iron-sulfur clusters in proteins involved in DNA synthesis and repair is. The research of the group revolves around proteins that are candidates to be the targets of new antiviral factors and to mediate DNA modifications and other important chemical transformations. These proteins often belong to large structural superfamilies with apparently similar representatives that are carrying out radically different reactions. We want to understand those functions and the extant determinants that endow this chemical versatility.
Courses Taught
| BCHM | 91g | Introduction to Research Practice |
| BCHM | 102a | Quantitative Approaches to Biochemical Systems |
Awards and Honors
Boosting the rational drug design of Hepatitis B Virus by large scale production of the X antigen (2018 - 2019)
Scholarship
Michelle Langton , Maria E Pandelia. "Hepatitis B Virus Oncoprotein HBx Is Not an ATPase." ACS Omega (2020): 16772-16778.
Michelle Langton, Sining Sun, Chie Ueda, Max Markey, Jiahua Chen, Isaac Paddy, Paul Jiang, Natalie Chin, Amy Milne and Maria-Eirini Pandelia. "The HD-Domain Metalloprotein Superfamily: An Apparent Common Protein Scaffold with Diverse Chemistries." Catalysts 10. 10 (2020): 1191.
Sining Sun, Maria-Eirini Pandelia. "HD-[HD-GYP] Phosphodiesterases: Activities and Evolutionary Diversification within the HD-GYP Family." Biochemistry (2020): 2340-2350.
Nitzan Koppel, Jordan E Bisanz, Maria-Eirini Pandelia, Peter J Turnbaugh, Emily P Balskus. "Discovery and characterization of a prevalent human gut bacterial enzyme sufficient for the inactivation of a family of plant toxins." (2019)
Pandelia, Maria-Eirini, Elizabeth C Wittenborn, Mériem Merrouch, Chie Ueda, Laura Fradale, Christophe Léger, Vincent Fourmond, Sébastien Dementin, Catherine L Drennan. "Redox-dependent rearrangements of the NiFeS cluster of carbon monoxide dehydrogenase." eLife (2019)
Rajakovich LJ, Pandelia ME, Mitchell AJ, Chang WC, Zhang B, Boal AK, Krebs C, Bollinger JM Jr.. "A New Microbial Pathway for Organophosphonate Degradation Catalyzed by Two Previously Misannotated Non-Heme-Iron Oxygenases.." (2019)
Richard Ayikpoe, Thacien Ngendahimana, Michelle Langton, Sheila Bonitatibus, Lindsey M. Walker, Sandra S. Eaton, Gareth R. Eaton, Maria Eirini-Pandelia, Sean J. Elliott, John A. Latham. "Spectroscopic and electrochemical characterization of the mycofactocin biosynthetic protein, MftC, provides insight into its redox flipping mechanism.." Biochemistry (2019): <10.1021/acs.biochem.8b01082>.
Rose HR, Maggiolo AO, McBride MJ, Palowitch GM, Pandelia ME, Davis KM, Yennawar NH, Boal AK.. "Structures of Class Id Ribonucleotide Reductase Catalytic Subunits Reveal a Minimal Architecture for Deoxynucleotide Biosynthesis.." (2019)
Kenney GE, Dassama LMK, Pandelia ME, Gizzi AS, Martinie RJ, Gao P, DeHart CJ, Schachner LF, Skinner OS, Ro SY, Zhu X, Sadek M, Thomas PM, Almo SC, Bollinger JM Jr., Krebs C, Kelleher NL, Rosenzweig AC. "The biosynthesis of Methanobactin." Science 359. 6382 (2018): 1411-1416.
Thomas C.Pochapsky, NathanWong, YihaoZhuang, JeffreyFutcher, Maria-EiriniPandelia, Drew R.Teitz, Allison M.Colthart. "NADH reduction of nitroaromatics as a probe for residual ferric form high-spin in a cytochrome P450." Biochim Biophys Acta 1866. 1 (2018): 126-133.
Clément Aussignargues, Maria-Eirini Pandelia, Markus Sutter, Jefferson S. Plegaria, Jan Zarzycki, Aiko Turmo, Jingcheng Huang, Daniel C. Ducat, Eric L. Hegg, Brian R. Gibney, and Cheryl A. Kerfeld. "Structure and Function of a Bacterial Microcompartment Shell Protein Engineered to Bind a [4Fe-4S] Cluster." Journal of American Chemical Society (2016)
Nicholas D. Lanz, Kyung-Hoon Lee, Abigail K. Horstmann, Maria-Eirini Pandelia, Robert M. Cicchillo, Carsten Krebs, and Squire J. Booker. "Characterization of Lipoyl Synthase from Mycobacterium tuberculosis." Biochemisstry (2016)
Cox, Nicholas, Nalepa, Anna, Pandelia, Maria-Eirini, Lubitz, Wolfgang, Savitsky, Anton. "Pulse Double-Resonance EPR Techniques for the Study of Metallobiomolecules." Methods in Enzymology. 1st ed. vol. 563 Ed. Peter Z. Qin and Kurt Warncke. Elsevier, 2015. 211-249.
Jessica-Hadj-Said, Maria-Eirini Pandelia, Christophe Leger, Vincent Fourmond, Sebastien Dementin. "CooC-Dependent Activation of the Carbon Monoxide Dehydrogenase (CooS) from Desulfovirio vulgaris." BBA Bioenergetics (2015)
Lauren J. Rajakovich, Hanne Nørgaard, Ning Li, Douglas Warui, Squire J. Booker, Carsten Krebs,J. Martin Bollinger, Jr., and Maria E. Pandelia. "Rapid Reduction of the Diferric-Peroxyhemiacetal Intermediate in Aldehyde-Deformylating Oxygenase by a Cyanobacterial Ferredoxin: Evidence for a Free-Radical Mechanism." Journal of American Chemical Society 137. 36 (2015)
Yixin Zhao, Nella M. Vargas-Barbosa, Megan E. Strayer, Nicholas S. McCool, Maria-Eirini Pandelia, Timothy P. Saunders, John R. Swierk, Juan F. Callejas, Lasse Jensen and Thomas E. Mallouk. "Understanding the effect of monomeric iridium(III/IV) aquo complexes on the photoelectrochemistry of IrOxnH2O–catalyzed water-splitting systems." Journal of American Chemical Society (2015)