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RBIF 109

Biological Sequence Analysis

This course provides a foundation in biological sequence analysis, including methods for handling next-generation sequencing data. Topics include genomic assembly and variant detection using short reads, methods for homology detection, functional annotation of sequences, and use of databases and visualization tools.

At the end of the course, students will be able to:

Align short reads to a reference genome.

Call variants using NGS data.

Evaluate the quality of a genomic assembly.

Search large databases for homologous sequences.

Annotate protein sequences using profile-based tools such as HMMer.

Identify functional features in genomic sequences, such as protein-coding sequences or small regulatory motifs.

Perform CRISPR guide sequence design.

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