Anticipate emergency situations, have proper handling equipment in the lab and readily available for spills. Check the MSDS to determine what is appropriate.
In the event of a spill or adverse reaction, notify lab personnel immediately that an incident has occurred. Do not attempt to handle a large spill/reaction/fire, or one in which you are not trained or equipped for. Turn off all ignition sources if this can be done safely; vacate the area and call for assistance.
Laboratory emergencies should be reported to the public safety office at 781-736-3333. Public safety will also contact the Office of Environmental Health and Safety at781-736-4262. Communicate the following:
- Location of spill/incident
- Type of material involved and quantity
- Injuries involved
- Fire/explosion
- Your location/contact information (or who to contact for further information)
Notify the principal investigator or designated safety officer as soon as possible.
If exposed to EB, skin/eye contact, immediately flush the eyes or skin with copious amounts of water for at least 15 minutes or until medical help arrives and you are instructed otherwise.
If EB dust is inhaled, move the victim to fresh air and contact public safety immediately for medical assistance.
Spills
Notify the Department of Environmental Health and Safety of significant ethidium-bromide spills.
Always wear full protective clothing, as described above, during any cleanup procedure. You should only clean up spills if you are aware of the hazards and have the proper safety and cleanup equipment.
A spill of EB powder can be carefully wiped up with wet paper towels the area decontaminated. Utilize UV light to locate the spill.
A spill of EB in liquid form should be absorbed with dry paper towels. Use a UV light source and illuminate the area to locate any remaining EB. All cleanup material should be placed in containers for proper disposal. DO NOT place EB-contaminated material in the regular trash.