Ruilin Irene Wang
Paradis Laboratory
Department of Biology
Brandeis University
Identifying Novel Rem2 Protein Interactors Using Yeast Two-Hybrid
Poster Abstract
Rem2 is the most highly expressed Ras-like GTPase in the brain and plays an important role in synaptic plasticity, a crucial function of the Central Nervous System (CNS). Typically, Ras-like GTPase are inactivated by GTPase activating proteins (GAP) and activated by guanine nucleotide exchange factors (GEF). To date, there are no identified GAPs and GEFs for Rem2. Using the S. Cerevisiae model, we performed Yeast Two-Hybrid (Y2H) by mating in order to detect and confirm more physiological Rem2 protein-protein interactions in the mouse brain. Previous data has shown that Rem2 binds to and inhibits the activity of CaMKII, which is a protein kinase and an important mediator of learning and memory. Preliminary results indicate that controls do not grow as expected, possibly due to poor protein-protein interactions in mated colonies. Future control experiments should be performed with fresh samples, or using a different approach such as co-transformation to conduct a reliable Y2H screen.
Personal Statement
Thanks to funding from the M.R. Bauer Foundation, I was able to work full- time in the Paradis lab this summer, after starting working on this project part-time in January of this year, and having previously worked in the same lab last summer. My experience in the Paradis Lab has been transformative for me both in terms of my personal and my professional growth. With my mentor’s help, I have not just developed proficient lab skills, but I have also gained a better understanding and perspective on the challenges of doing research, in particular the patience and critical thinking it takes when unexpected results arise. I have also learned how to communicate more effectively within a lab environment and how to read, process and utilize primary literature better in the field of neuroscience. In reflecting upon my work this summer, I have also decided to adjust my courses for my upcoming senior year. Furthermore, with my poster project and presentation, I’ve had the opportunity to practice my writing and public speaking skills in the context of scientific research, something I believe that will be of great value as I move forward to graduate school.