## Effects of Confinement Geometry on Active Matter

Interactions between cellular components and the cell membrane play a key role in many cellular functions.

For example, in a Drosophila oocyte, membrane confinement organizes molecular-motor-driven motions of cytoplasmic contents to produce the spontaneous circulation known as cytoplasmic streaming [1]. Boundaries also influence, and are influenced by, collective behaviors of cells. For example, swimming bacteria can be concentrated by funnel-shaped boundaries, while the motions of a bacterial bath can turn microscopic gears [2, 3]. Boundaries are necessarily present in any real-world active matter system, and these examples illustrate that certain sizes or shapes of boundaries can dramatically alter the dynamics of active systems. Yet, most theoretical active matter research has neglected boundaries, and there is currently no general theory for the effect of boundary geometry.

Motivated by this need, Yaouen Fily developed a statistical theory for non-aligning and non-interacting self-propelled particles confined to convex containers [4]. The theory and computer simulations showed that confinement dramatically affects the behavior of self-propelled particles. Below a threshold container size, the active particles are confined to the boundary, with a density that is inversely proportional to, and a pressure that decays exponentially with, the local boundary curvature (Fig. 1A,C). We have now extended the theory to also describe non-convex containers [5] (Fig. 1B). Thus, the theory now allows predicting particle densities for any box shape, and also enables designing a box shape to realize virtually any desired particle distribution. We have now extended the analysis to 3D (Fig. 1D), where we find that the particle density is proportional to the local Gaussian curvature of the boundary.

### References

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*Dynamics and density distribution of strongly confined noninteracting nonaligning self-propelled particles in a nonconvex boundary.*Physical Review E, 2015.**91**(1): 012125.