"Charizard:" Abberior Facility Line 3D STED
This fully evolved stimulated emission depletion super-resolution point-scanner comes with a number of amazing features to help you resolve the smallest of details. It is capable of resolving 2D structures down to 30nm or 3D STED to 60nm. With careful sample preparation, you’ll be amazed how easy it is to get incredible resolution on this system.
Funded by an NIH S10 Instrumentation Grant ID - 1S10OD034223-01 this system is available to internal and external users.
Scope Body
Olympus IX83 Inverted
Lasers
- 405
- 488
- 561
- 640
- Depletion Laser - 775nm
Objectives (Mag/NA)
- Air:
- 10x/0.45
- 20x/0.8 PlanApo
- Immersion:
- 60x XPlanApo 1.25 Water
- 60x XPlanApo 1.35 Sil
- 60x XPlanApo 1.42 Oil
Detectors
- Spectral detection across all detectors
- 3x APDs
- 1 Array Detector APD
Environmental Control
Full temperature regulated enclosure
Sample Holders
Slides, plates, dishes
Features
- Adaptive optics deformable mirror for refractive index mismatch correction
- Matrix detector for pixel reassignment and noise reduction
- Fluorescence Lifetime Imaging
- Adaptive Illumination for reducing unnecessary exposure of samples
- Easy auto-alignment procedure
- Intuitive software with excellent data management functionality